Overall biochemical changes in bacteria photosensitized with cationic porphyrins monitored by infrared spectroscopy.
Future Med Chem
; 8(6): 613-28, 2016 04.
Article
in En
| MEDLINE
| ID: mdl-27073984
ABSTRACT
BACKGROUND:
Photodynamic inactivation of micro-organisms is a promising nonantibiotic multitarget approach to treat localized and superficial infections through oxidative stress. Herein, the changes occurring on major cellular components of Escherichia coli and Staphylococcus warneri, induced by photosensitization with cationic porphyrins (Tri-Py(+)-Me-PF and Tetra-Py(+)-Me) and white light, were monitored by infrared spectroscopy.RESULTS:
In E. coli, most of the changes occurred on proteins and lipids, suggesting a key effect on lipopolysaccharides in the first irradiation times. In S. warneri, proteins were the major molecular targets of oxidative damage but phospholipids and polysaccharides were also affected.CONCLUSION:
Infrared spectroscopy is a very interesting tool to monitor biochemical changes induced by photosensitization in bacteria and also to infer on its mechanism of action.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Porphyrins
/
Staphylococcus
/
Photosensitizing Agents
/
Escherichia coli
/
Light
Language:
En
Journal:
Future Med Chem
Year:
2016
Type:
Article
Affiliation country:
Portugal