Subnuclear localization, rates and effectiveness of UVC-induced unscheduled DNA synthesis visualized by fluorescence widefield, confocal and super-resolution microscopy.

Pierzynska-Mach, Agnieszka; Szczurek, Aleksander; Cella Zanacchi, Francesca; Pennacchietti, Francesca; Drukala, Justyna; Diaspro, Alberto; Cremer, Christoph; Darzynkiewicz, Zbigniew; Dobrucki, Jurek W

Pierzynska-Mach, Agnieszka; Szczurek, Aleksander; Cella Zanacchi, Francesca; Pennacchietti, Francesca; Drukala, Justyna; Diaspro, Alberto; Cremer, Christoph; Darzynkiewicz, Zbigniew; Dobrucki, Jurek W.

Affiliation

Pierzynska-Mach A; a Laboratory of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University , Kraków , Poland.
Szczurek A; b Institute of Molecular Biology , Mainz , Germany.
Cella Zanacchi F; c Nanoscopy, Istituto Italiano di Tecnologia , Genova , Italy.
Pennacchietti F; c Nanoscopy, Istituto Italiano di Tecnologia , Genova , Italy.
Drukala J; d Department of Cell Biology , Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University , Kraków , Poland.
Diaspro A; c Nanoscopy, Istituto Italiano di Tecnologia , Genova , Italy.
Cremer C; b Institute of Molecular Biology , Mainz , Germany.
Darzynkiewicz Z; e Brander Cancer Research Institute and Department of Pathology, New York Medical College , Valhalla , NY , USA.
Dobrucki JW; a Laboratory of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University , Kraków , Poland.

Cell Cycle ; 15(8): 1156-67, 2016.

Article in En | MEDLINE | ID: mdl-27097376

Subject(s)

Cell Nucleus/metabolism; DNA/biosynthesis; Microscopy, Confocal/methods; Ultraviolet Rays; Bromodeoxyuridine/metabolism; Cell Nucleus/radiation effects; Cells, Cultured; Deoxyuridine/analogs & derivatives; Deoxyuridine/metabolism; Dose-Response Relationship, Radiation; Fibroblasts/metabolism; Fibroblasts/radiation effects; Fluorescence; Humans; Nucleic Acid Denaturation/radiation effects; Subcellular Fractions/metabolism; Subcellular Fractions/radiation effects; Time Factors

Key words

BrdU; DNA damage; DNA repair; EdU; NER; UDS; confocal microscopy; cytometry; dSTORM; fluorescence; nucleotide excision repair; single molecule localization microscopy; super-resolution microscopy; ultraviolet light; unscheduled DNA synthesis

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ultraviolet Rays / DNA / Cell Nucleus / Microscopy, Confocal Limits: Humans Language: En Journal: Cell Cycle Year: 2016 Type: Article Affiliation country: Poland

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