Two unexpected promiscuous activities of the iron-sulfur protein IspH in production of isoprene and isoamylene.

Ge, Deyong; Xue, Yanfen; Ma, Yanhe

Ge, Deyong; Xue, Yanfen; Ma, Yanhe.

Affiliation

Ge D; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.
Xue Y; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
Ma Y; College of Medicine, Anhui University of Science and Technology, Huainan, People's Republic of China.

Microb Cell Fact ; 15: 79, 2016 May 11.

Article in En | MEDLINE | ID: mdl-27169371

Subject(s)

Bacillus/metabolism; Bacterial Proteins/metabolism; Hemiterpenes/biosynthesis; Iron-Sulfur Proteins/metabolism; Bacillus/growth & development; Bacterial Proteins/genetics; Butadienes; Electrophoresis, Polyacrylamide Gel; Ferredoxins/metabolism; Gas Chromatography-Mass Spectrometry; Hemiterpenes/chemistry; Hemiterpenes/metabolism; Iron-Sulfur Proteins/genetics; Isomerism; Mutagenesis, Site-Directed; Organophosphates/chemistry; Organophosphates/metabolism; Organophosphorus Compounds/chemistry; Organophosphorus Compounds/metabolism; Oxidoreductases/genetics; Oxidoreductases/metabolism; Pentanes; Recombinant Proteins/biosynthesis; Recombinant Proteins/chemistry; Recombinant Proteins/isolation & purification

Key words

(E)-4-Hydroxy-3-methyl-2-butenyl diphosphate (HMBPP); 2-Methyl-2-butene; 3-Methyl-1-butene; Bacillus; Dimethylallyl diphosphate (DMAPP); Isoprene; Isoprene synthase; IspH; IspS

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacillus / Bacterial Proteins / Hemiterpenes / Iron-Sulfur Proteins Language: En Journal: Microb Cell Fact Journal subject: BIOTECNOLOGIA / MICROBIOLOGIA Year: 2016 Type: Article

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