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Connective tissue growth factor is activated by gastrin and involved in gastrin-induced migration and invasion.
Bhandari, Sabin; Bakke, Ingunn; Kumar, J; Beisvag, Vidar; Sandvik, Arne K; Thommesen, Liv; Varro, Andrea; Nørsett, Kristin G.
Affiliation
  • Bhandari S; Department of Cancer Research and Molecular Medicine, NTNU, Norwegian University of Science and Technology, Trondheim, Norway.
  • Bakke I; Department of Cancer Research and Molecular Medicine, NTNU, Norwegian University of Science and Technology, Trondheim, Norway.
  • Kumar J; Department of Cell and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom.
  • Beisvag V; Department of Cancer Research and Molecular Medicine, NTNU, Norwegian University of Science and Technology, Trondheim, Norway.
  • Sandvik AK; Department of Cancer Research and Molecular Medicine, NTNU, Norwegian University of Science and Technology, Trondheim, Norway; Department of Gastroenterology and Hepatology, St. Olav's University Hospital, Trondheim, Norway.
  • Thommesen L; Department of Cancer Research and Molecular Medicine, NTNU, Norwegian University of Science and Technology, Trondheim, Norway.
  • Varro A; Department of Cell and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom.
  • Nørsett KG; Department of Cancer Research and Molecular Medicine, NTNU, Norwegian University of Science and Technology, Trondheim, Norway; Central Norway Regional Health Authority (RHA), Stjørdal, Norway. Electronic address: kristin.norsett@ntnu.no.
Biochem Biophys Res Commun ; 475(1): 119-24, 2016 06 17.
Article in En | MEDLINE | ID: mdl-27179776
ABSTRACT
Connective tissue growth factor (CTGF) has been reported in gastric adenocarcinoma and in carcinoid tumors. The aim of this study was to explore a possible link between CTGF and gastrin in gastric epithelial cells and to study the role of CTGF in gastrin induced migration and invasion of AGS-GR cells. The effects of gastrin were studied using RT-qPCR, Western blot and assays for migration and invasion. We report an association between serum gastrin concentrations and CTGF abundancy in the gastric corpus mucosa of hypergastrinemic subjects and mice. We found a higher expression of CTGF in gastric mucosa tissue adjacent to tumor compared to normal control tissue. We showed that gastrin induced expression of CTGF in gastric epithelial AGS-GR cells via MEK, PKC and PKB/AKT pathways. CTGF inhibited gastrin induced migration and invasion of AGS-GR cells. We conclude that CTGF expression is stimulated by gastrin and involved in remodeling of the gastric epithelium.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Stomach / Stomach Neoplasms / Gastrins / Adenocarcinoma / Connective Tissue Growth Factor Limits: Animals / Humans Language: En Journal: Biochem Biophys Res Commun Year: 2016 Type: Article Affiliation country: Norway
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Stomach / Stomach Neoplasms / Gastrins / Adenocarcinoma / Connective Tissue Growth Factor Limits: Animals / Humans Language: En Journal: Biochem Biophys Res Commun Year: 2016 Type: Article Affiliation country: Norway