Identification of the High-affinity Substrate-binding Site of the Multidrug and Toxic Compound Extrusion (MATE) Family Transporter from Pseudomonas stutzeri.

Nie, Laiyin; Grell, Ernst; Malviya, Viveka Nand; Xie, Hao; Wang, Jingkang; Michel, Hartmut

Nie, Laiyin; Grell, Ernst; Malviya, Viveka Nand; Xie, Hao; Wang, Jingkang; Michel, Hartmut.

Affiliation

Nie L; From the Tianjin University, School of Chemical Engineering and Technology, State Key Laboratory for Chemical Engineering, Collaborative Innovation Center of Chemical Science and Chemical Engineering, 300072 Tianjin, China and the Department of Molecular Membrane Biology, Max Planck Institute of Bio
Grell E; the Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max-von-Laue-Strasse 3, 60438 Frankfurt am Main, Germany.
Malviya VN; the Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max-von-Laue-Strasse 3, 60438 Frankfurt am Main, Germany.
Xie H; the Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max-von-Laue-Strasse 3, 60438 Frankfurt am Main, Germany.
Wang J; From the Tianjin University, School of Chemical Engineering and Technology, State Key Laboratory for Chemical Engineering, Collaborative Innovation Center of Chemical Science and Chemical Engineering, 300072 Tianjin, China and wangjkch@tju.edu.cn.
Michel H; the Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max-von-Laue-Strasse 3, 60438 Frankfurt am Main, Germany hartmut.michel@biophys.mpg.de.

J Biol Chem ; 291(30): 15503-14, 2016 07 22.

Article in En | MEDLINE | ID: mdl-27235402

Subject(s)

Bacterial Proteins/chemistry; Carrier Proteins/chemistry; Drug Resistance, Multiple, Bacterial; Indoles/chemistry; Pseudomonas stutzeri/chemistry; Amino Acid Substitution; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Carrier Proteins/genetics; Carrier Proteins/metabolism; Indoles/metabolism; Mutation, Missense; Pseudomonas stutzeri/genetics; Pseudomonas stutzeri/metabolism

Key words

DAPI-binding site; binding affinity; isothermal titration calorimetry (ITC); ligand-binding protein; membrane biophysics; membrane protein; multidrug transporter

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Carrier Proteins / Drug Resistance, Multiple, Bacterial / Pseudomonas stutzeri / Indoles Type of study: Diagnostic_studies Language: En Journal: J Biol Chem Year: 2016 Type: Article

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