Your browser doesn't support javascript.
loading
Varicella zoster virus infection of human fetal lung cells alters mitochondrial morphology.
Keller, Amy C; Badani, Hussain; McClatchey, P Mason; Baird, Nicholas L; Bowlin, Jacqueline L; Bouchard, Ron; Perng, Guey-Chuen; Reusch, Jane E B; Kaufer, Benedikt B; Gilden, Don; Shahzad, Aamir; Kennedy, Peter G E; Cohrs, Randall J.
Affiliation
  • Keller AC; Division of Endocrinology, University of Colorado School of Medicine, Aurora, 80045, CO, USA.
  • Badani H; Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th Avenue, Box B182, Aurora, 80045, CO, USA.
  • McClatchey PM; Division of Endocrinology, University of Colorado School of Medicine, Aurora, 80045, CO, USA.
  • Baird NL; Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th Avenue, Box B182, Aurora, 80045, CO, USA.
  • Bowlin JL; Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th Avenue, Box B182, Aurora, 80045, CO, USA.
  • Bouchard R; Department of Medicine, Denver VA Medical Center, Denver, 80220, CO, USA.
  • Perng GC; Department of Microbiology and Immunology, College of Medicine, and Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan.
  • Reusch JE; Division of Endocrinology, University of Colorado School of Medicine, Aurora, 80045, CO, USA.
  • Kaufer BB; Department of Medicine, Denver VA Medical Center, Denver, 80220, CO, USA.
  • Gilden D; Institut für Virologie, Freie Universität, Berlin, Germany.
  • Shahzad A; Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th Avenue, Box B182, Aurora, 80045, CO, USA.
  • Kennedy PG; Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, 80045, CO, USA.
  • Cohrs RJ; Department of Biomolecular Structural Chemistry, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
J Neurovirol ; 22(5): 674-682, 2016 10.
Article in En | MEDLINE | ID: mdl-27245593
Varicella zoster virus (VZV) is a ubiquitous alphaherpesvirus that establishes latency in ganglionic neurons throughout the neuraxis after primary infection. Here, we show that VZV infection induces a time-dependent significant change in mitochondrial morphology, an important indicator of cellular health, since mitochondria are involved in essential cellular functions. VZV immediate-early protein 63 (IE63) was detected in mitochondria-rich cellular fractions extracted from infected human fetal lung fibroblasts (HFL) by Western blotting. IE63 interacted with cytochrome c oxidase in bacterial 2-hybrid analyses. Confocal microscopy of VZV-infected HFL cells at multiple times after infection revealed the presence of IE63 in the nucleus, mitochondria, and cytoplasm. Our data provide the first evidence that VZV infection induces alterations in mitochondrial morphology, including fragmentation, which may be involved in cellular damage and/or death during virus infection.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Viral Envelope Proteins / Immediate-Early Proteins / Electron Transport Complex IV / Herpesvirus 3, Human / Host-Pathogen Interactions / Fibroblasts / Mitochondria Limits: Humans Language: En Journal: J Neurovirol Journal subject: NEUROLOGIA / VIROLOGIA Year: 2016 Type: Article Affiliation country: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Viral Envelope Proteins / Immediate-Early Proteins / Electron Transport Complex IV / Herpesvirus 3, Human / Host-Pathogen Interactions / Fibroblasts / Mitochondria Limits: Humans Language: En Journal: J Neurovirol Journal subject: NEUROLOGIA / VIROLOGIA Year: 2016 Type: Article Affiliation country: United States