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Visualizing Cancer Cell Chemotaxis and Invasion in 2D and 3D.
Susanto, Olivia; Muinonen-Martin, Andrew J; Nobis, Max; Insall, Robert H.
Affiliation
  • Susanto O; CR-UK Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.
  • Muinonen-Martin AJ; Melanoma Clinic, Leeds Cancer Centre, St. James's University Hospital, Beckett Street, Leeds, LS9 7TF, UK.
  • Nobis M; CR-UK Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.
  • Insall RH; CR-UK Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK. r.insall@beatson.gla.ac.uk.
Methods Mol Biol ; 1407: 217-28, 2016.
Article in En | MEDLINE | ID: mdl-27271906
ABSTRACT
We describe three chemotaxis assays-Insall chambers, circular invasion assays, and 3D organotypic assays-that are particularly appropriate for measuring migration of cancer cells in response to gradients of soluble attractants. Each assay has defined advantages, and together they provide the best possible quantitative assessment of cancer chemotaxis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Movement / Chemotaxis / Neoplasms Limits: Animals / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2016 Type: Article Affiliation country: United kingdom
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Movement / Chemotaxis / Neoplasms Limits: Animals / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2016 Type: Article Affiliation country: United kingdom