[Metabolites Identification for Alkaloids from Nelumbinis Plumula in Caco-2 Cells by LC/MS/MS].
Zhong Yao Cai
; 38(12): 2531-4, 2015 Dec.
Article
in Zh
| MEDLINE
| ID: mdl-27352532
ABSTRACT
OBJECTIVE:
To identify the metabolites of norcoclaurine,liensinine, isoliensinine and neferine in Caco-2 cells by LC/ MS/MS.METHODS:
After Caco-2 cells were treated with norcoclaurine, liensinine, isoliensinine or neferine for 3, 6 and 12 h, samples were collected, purified and then analyzed by LC/MS/MS. The structures of the metabolites were elucidated by molecular masses, retention times, MS and MS/MS spectra comparing with those of the parent drug.RESULTS:
The procedure identified that the major metabolites of norcoclaurine were methylnorcoclaurine and norcoclaurine-glucuronide, the major metabolite of liensinine was demethyl-liensinine, the major metabolite of isoliensinine was demethyl-isoliensinine, the major metabolites of neferine were liensinine, isoliensinine and their further demethylation products.CONCLUSION:
LC/MS/MS is simple, rapid and sensitive for the metabolites identification. Methylation, demethylation and glucuronidation are main metabolic pathways of alkaloids from Nelumbinis Plumula in Caco-2 cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Drugs, Chinese Herbal
/
Magnoliopsida
/
Alkaloids
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
Zh
Journal:
Zhong Yao Cai
Journal subject:
TERAPIAS COMPLEMENTARES
Year:
2015
Type:
Article