RecA stimulates AlkB-mediated direct repair of DNA adducts.
Nucleic Acids Res
; 44(18): 8754-8763, 2016 Oct 14.
Article
in En
| MEDLINE
| ID: mdl-27378775
ABSTRACT
The Escherichia coli AlkB protein is a 2-oxoglutarate/Fe(II)-dependent demethylase that repairs alkylated single stranded and double stranded DNA. Immunoaffinity chromatography coupled with mass spectrometry identified RecA, a key factor in homologous recombination, as an AlkB-associated protein. The interaction between AlkB and RecA was validated by yeast two-hybrid assay; size-exclusion chromatography and standard pull down experiment and was shown to be direct and mediated by the N-terminal domain of RecA. RecA binding results AlkB-RecA heterodimer formation and RecA-AlkB repairs alkylated DNA with higher efficiency than AlkB alone.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rec A Recombinases
/
DNA Adducts
/
DNA Repair
/
AlkB Enzymes
Type of study:
Prognostic_studies
Language:
En
Journal:
Nucleic Acids Res
Year:
2016
Type:
Article
Affiliation country:
India