Expression of macrophage migration inhibitory factor (MIF) in bovine oviducts is higher in the postovulatory phase than during the oestrus and luteal phase.
Reprod Fertil Dev
; 29(8): 1521-1529, 2017 Aug.
Article
in En
| MEDLINE
| ID: mdl-27465152
ABSTRACT
Whether macrophage migration inhibitory factor (MIF) in the bovine oviduct is important for early embryogenesis has not been well substantiated. The aim of the present study was to test the hypothesis that bovine oviduct expresses higher levels of MIF during the post-ovulation phase. Both ampullary and isthmic samples were collected from Japanese black heifers during oestrus (Day 0; n=5), postovulation (Day 3; n=6) and luteal phase (Days 9-12; n=5). MIF mRNA and protein were extracted from the ampullary and isthmic samples and their levels measured by real-time polymerase chain reaction and western blot analysis respectively. Fluorescent immunohistochemistry was performed on frozen ampullary and isthmic sections using antibodies against MIF. MIF mRNA and protein expression was higher in the postovulatory phase than during oestrus and the luteal phase (P<0.05). Fluorescent immunohistochemistry confirmed that in all phases of the oestrous cycle evaluated, the primary site of MIF expression in the ampulla and isthmus was the tunica mucosa. In conclusion, the bovine ampulla and isthmus have higher MIF expression during the postovulatory phase. Further studies are needed to clarify the role of MIF in bovine oviducts.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oviducts
/
Ovulation
/
Estrus
/
Macrophage Migration-Inhibitory Factors
/
Luteal Phase
Limits:
Animals
Language:
En
Journal:
Reprod Fertil Dev
Journal subject:
MEDICINA REPRODUTIVA
Year:
2017
Type:
Article
Affiliation country:
Japan