Pre-embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells.

Boykins, Lou G; Jones, Jonathan C R; Estraño, Carlos E; Schwartzbach, Steven D; Skalli, Omar

Boykins, Lou G; Jones, Jonathan C R; Estraño, Carlos E; Schwartzbach, Steven D; Skalli, Omar.

Affiliation

Boykins LG; Department of Biological Sciences, The University of Memphis, 103 Life Sciences Bldg, Memphis, TN, 38152, USA.
Jones JC; School of Molecular Biosciences, Washington State University, BLS 202F, 1770 NE Stadium Way, Pullman, WA, 99164, USA.
Estraño CE; Department of Biological Sciences, The University of Memphis, 103 Life Sciences Bldg, Memphis, TN, 38152, USA.
Schwartzbach SD; Department of Biological Sciences, The University of Memphis, 103 Life Sciences Bldg, Memphis, TN, 38152, USA.
Skalli O; Department of Biological Sciences, The University of Memphis, 103 Life Sciences Bldg, Memphis, TN, 38152, USA. oskalli@memphis.edu.

Methods Mol Biol ; 1474: 217-32, 2016.

Article in En | MEDLINE | ID: mdl-27515083

Subject(s)

Endothelial Cells/ultrastructure; Immunohistochemistry/methods; Integrin beta3/genetics; Microscopy, Immunoelectron/methods; Tissue Embedding/methods; Vimentin/genetics; Antibodies/chemistry; Antigens/genetics; Antigens/metabolism; Cell Line; Endothelial Cells/metabolism; Epoxy Resins/chemistry; Gene Expression; Humans; Integrin beta3/metabolism; Microtomy; Staining and Labeling/methods; Tissue Fixation/methods; Vimentin/metabolism

Key words

Antibodies; Immunogold; Monolayer; Pre-embedding

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Vimentin / Immunohistochemistry / Microscopy, Immunoelectron / Tissue Embedding / Integrin beta3 / Endothelial Cells Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2016 Type: Article Affiliation country: United States

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