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The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots.
Siciliano, Camilla; Bordin, Antonella; Ibrahim, Mohsen; Chimenti, Isotta; Cassiano, Francesco; Gatto, Ilenia; Mangino, Giorgio; Coccia, Andrea; Miglietta, Selenia; Bastianelli, Daniela; Petrozza, Vincenzo; Calogero, Antonella; Frati, Giacomo; De Falco, Elena.
Affiliation
  • Siciliano C; Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: cami.siciliano@gmail.com.
  • Bordin A; Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: anto.bordin@libero.it.
  • Ibrahim M; Division of Thoracic Surgery, Department of Medical-Surgical Science and Translational Medicine, Sapienza University of Rome, S. Andrea Hospital, via di Grottarossa 1035, 00189 Rome, Italy. Electronic address: mohsen.ibrahim@uniroma1.it.
  • Chimenti I; Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: isotta.chimenti@uniroma1.it.
  • Cassiano F; Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Section of Anatomy, Electron Microscopy Unit, Laboratory "Pietro M. Motta", Sapienza University of Rome, Rome, Italy. Electronic address: fra.cassiano@gmail.com.
  • Gatto I; Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: ilenia.gatto@uniroma1.it.
  • Mangino G; Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: gmangino@libero.it.
  • Coccia A; Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: andrea.coccia@uniroma1.it.
  • Miglietta S; Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Section of Anatomy, Electron Microscopy Unit, Laboratory "Pietro M. Motta", Sapienza University of Rome, Rome, Italy. Electronic address: selenia.miglietta@uniroma1.it.
  • Bastianelli D; Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: daniela.bastianelli@uniroma1.it.
  • Petrozza V; Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: vincenzo.petrozza@uniroma1.it.
  • Calogero A; Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: antonella.calogero@uniroma1.it.
  • Frati G; Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy; Department of AngioCardioNeurology, IRCCS NeuroMed, 86077 Pozzilli, IS, Italy. Electronic address: fraticello@inwind.it.
  • De Falco E; Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: elena.defalco@uniroma1.it.
Stem Cell Res ; 17(2): 342-351, 2016 09.
Article in En | MEDLINE | ID: mdl-27614132
ABSTRACT
Indirect evidence suggests that adipose tissue-derived stromal cells (ASCs) possess different physiological and biological variations related to the anatomical localization of the adipose depots. Accordingly, to investigate the influence of the tissue origin on the intrinsic properties of ASCs and to assess their response to specific stimuli, we compared the biological, functional and ultrastructural properties of two ASC pools derived from mediastinal and subcutaneous depots (thoracic compartment) by means of supplements such as platelet lysate (PL) and FBS. Subcutaneous ASCs exhibited higher proliferative and clonogenic abilities than mediastinal counterpart, as well as increased secreted levels of IL-6 combined with lower amount of VEGF-C. In contrast, mediastinal ASCs displayed enhanced pro-angiogenic and adipogenic differentiation properties, increased cell diameter and early autophagic processes, highlighted by electron microscopy. Our results further support the hypothesis that the origin of adipose tissue significantly defines the biological properties of ASCs, and that a homogeneric function for all ASCs cannot be assumed.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Stromal Cells / Subcutaneous Fat Limits: Aged / Female / Humans / Male / Middle aged Language: En Journal: Stem Cell Res Year: 2016 Type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Stromal Cells / Subcutaneous Fat Limits: Aged / Female / Humans / Male / Middle aged Language: En Journal: Stem Cell Res Year: 2016 Type: Article