Cell adhesion molecules are altered during irinotecan-induced mucositis: a qualitative histopathological study.
Support Care Cancer
; 25(2): 391-398, 2017 02.
Article
in En
| MEDLINE
| ID: mdl-27650103
ABSTRACT
PURPOSE:
Chemotherapy-induced mucositis is characterised by damage to mucous membranes throughout the alimentary tract. This study aims to investigate the expression of cell adhesion molecules (CAMs) following treatment with irinotecan.METHODS:
Dark agouti rats received a single dose of 175 mg/kg irinotecan and sacrificed at various time points after treatment. Picro-sirius red staining indicated an increase in collagen around crypts from 24 h in both small and large intestinal regions and this diminished at the later time points. CAMs E-cadherin, P-selectin, E-selectin and integrin-α1 were examined using immunohistochemistry.RESULTS:
E-cadherin was significantly elevated in jejunal crypts at the time of maximal tissue damage (48 h), while it decreased at the healing phase (96 h) in both jejunum and colon. P-selectin expression decreased significantly in the jejunum following irinotecan. Crypt expression of E-selectin was significantly elevated in the healing phase of mucositis (96 h). Integrin-α1 expression was significantly altered during the time course in the villus (p = 0.0032) and lamina propria (p = 0.039).CONCLUSIONS:
Irinotecan induced a significant alteration in CAM expression in the jejunum and colon. Changes in adhesion molecule expression may have a direct impact on the loss of mucosal layer integrity seen in mucositis.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Camptothecin
/
Immunohistochemistry
/
Cell Adhesion Molecules
/
Mucositis
/
Intestinal Mucosa
/
Jejunum
/
Antineoplastic Agents, Phytogenic
Type of study:
Qualitative_research
Limits:
Animals
Language:
En
Journal:
Support Care Cancer
Journal subject:
NEOPLASIAS
/
SERVICOS DE SAUDE
Year:
2017
Type:
Article
Affiliation country:
Australia