Fto-Deficiency Affects the Gene and MicroRNA Expression Involved in Brown Adipogenesis and Browning of White Adipose Tissue in Mice.
Int J Mol Sci
; 17(11)2016 Nov 07.
Article
in En
| MEDLINE
| ID: mdl-27827997
ABSTRACT
Genetic variants in the fat mass- and obesity-associated gene Fto are linked to the onset of obesity in humans. The causal role of the FTO protein in obesity is supported by evidence obtained from transgenic mice; however, the underlying molecular pathways pertaining to the role of FTO in obesity have yet to be established. In this study, we investigate the Fto gene in mouse brown adipose tissue and in the browning process of white adipose tissue. We analyze distinct structural and molecular factors in brown and white fat depots of Fto-deficient mice under normal and obesogenic conditions. We report significant alterations in the morphology of adipose tissue depots and the expression of mRNA and microRNA related to brown adipogenesis and metabolism in Fto-deficient mice. Furthermore, we show that high-fat feeding does not attenuate the browning process of Fto-deficient white adipose tissue as observed in wild-type tissue, suggesting a triggering effect of the FTO pathways by the dietary environment.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adipose Tissue, Brown
/
MicroRNAs
/
Adipogenesis
/
Adipose Tissue, White
/
Alpha-Ketoglutarate-Dependent Dioxygenase FTO
/
Obesity
Type of study:
Etiology_studies
Limits:
Animals
Language:
En
Journal:
Int J Mol Sci
Year:
2016
Type:
Article
Affiliation country:
Finland