Measurement of Effector Protein Translocation Using Phosphorylatable Epitope Tags and Phospho-Specific Antibodies.

Bartra, Sara Schesser; Plano, Gregory V

Bartra, Sara Schesser; Plano, Gregory V.

Affiliation

Bartra SS; Department of Microbiology and Immunology, Miller School of Medicine, University of Miami, 1600 NW 10th Ave., Miami, FL, 33136, USA.
Plano GV; Department of Microbiology and Immunology, Miller School of Medicine, University of Miami, 1600 NW 10th Ave., Miami, FL, 33136, USA. gplano@med.miami.edu.

Methods Mol Biol ; 1531: 111-119, 2017.

Article in En | MEDLINE | ID: mdl-27837486

Subject(s)

Antibodies, Phospho-Specific; Bacterial Proteins/metabolism; Bacterial Secretion Systems/metabolism; Epitopes; Recombinant Fusion Proteins/metabolism; Amino Acid Sequence; Animals; Antibodies, Phospho-Specific/immunology; Bacterial Proteins/chemistry; Blotting, Western; Cell Line; Epitopes/chemistry; Epitopes/immunology; Gene Order; Mice; Phosphorylation; Plasmids/genetics; Protein Transport; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/immunology; Yersinia pestis/immunology; Yersinia pestis/metabolism

Key words

Effector proteins; Phospho-specific antibodies; Translocation; Type III secretion; Type IV secretion; Yersinia pestis

Search on Google

XML

PubMed Links

Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Recombinant Fusion Proteins / Antibodies, Phospho-Specific / Bacterial Secretion Systems / Epitopes Limits: Animals Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2017 Type: Article Affiliation country: United States

Search on Google

XML

PubMed Links