Potential effects of samsum ant, Brachyponera sennaarensis, venom on TNF-α/NF-κB mediated inflammation in CCL4-toxicity in vivo.
Lipids Health Dis
; 15(1): 198, 2016 Nov 18.
Article
in En
| MEDLINE
| ID: mdl-27863485
ABSTRACT
BACKGROUND:
Ant venom shows antimicrobial, anti-parasitic and anti-inflammatory activities, both in vitro and in vivo. Our recent studies have confirmed the role of samsum ant venom (SAV) as a powerful antioxidant. This study aimed to investigate whether SAV as a potential treatment for CCl4-induced acute liver toxicity in an animal (rat) model.METHODS:
Thirty-two rats were assigned into four groups; the first one served as the control. The second group received a single dose of 1 ml/kg CCl4 in a 11 ratio with olive oil through an intraperitoneal injection. The third group received a single dose of 1 ml/kg CCl4 and then treated with SAV at a dose of 100 µg SAV twice a week for three weeks. The fourth group received a dose of 100 µg SAV only twice a week for three weeks. ELISA, RT-PCR and histopathological examinations were applied.RESULTS:
Results showed that antioxidant enzymes were significantly reduced in the diseased animals. SAV was found to significantly restore the oxidative stability in diseased animals. ELISA estimation and RT-PCR analysis also showed significant upregulation of both nuclear factor (κB) NF-κB and inhibitor (κB) IκB, respectively, in the diseased animals compared to the normal ones. The expression of tumour necrosis factor alpha (TNF-α) and pro-apoptotic receptor (Fas) were also significantly up-regulated in the diseased rats. Interestingly, SAV was found to significantly restore NF-κB, IκB and TNF-α in the diseased rats to the normal values. As a result, liver enzymes, serum proteins and lipid concentrations were significantly improved by SAV in CCl4-animals in comparison with the control ones. Moreover, SAV obviously improved the hepatic tissues of the same group was.CONCLUSION:
SAV treatment restores the normal biochemical and oxidative stability by improving the TNF-α/NF-κB mediated inflammation in CCL4-treated rats.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carbon Tetrachloride
/
Chemical and Drug Induced Liver Injury
/
Inflammation
/
Ant Venoms
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Lipids Health Dis
Journal subject:
BIOQUIMICA
/
METABOLISMO
Year:
2016
Type:
Article
Affiliation country:
Saudi Arabia