Mycobacterium tuberculosis replicates within necrotic human macrophages.
J Cell Biol
; 216(3): 583-594, 2017 03 06.
Article
in En
| MEDLINE
| ID: mdl-28242744
ABSTRACT
Mycobacterium tuberculosis modulation of macrophage cell death is a well-documented phenomenon, but its role during bacterial replication is less characterized. In this study, we investigate the impact of plasma membrane (PM) integrity on bacterial replication in different functional populations of human primary macrophages. We discovered that IFN-γ enhanced bacterial replication in macrophage colony-stimulating factor-differentiated macrophages more than in granulocyte-macrophage colony-stimulating factor-differentiated macrophages. We show that permissiveness in the different populations of macrophages to bacterial growth is the result of a differential ability to preserve PM integrity. By combining live-cell imaging, correlative light electron microscopy, and single-cell analysis, we found that after infection, a population of macrophages became necrotic, providing a niche for M. tuberculosis replication before escaping into the extracellular milieu. Thus, in addition to bacterial dissemination, necrotic cells provide first a niche for bacterial replication. Our results are relevant to understanding the environment of M. tuberculosis replication in the host.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA Replication
/
Macrophages
/
Mycobacterium tuberculosis
/
Necrosis
Limits:
Humans
Language:
En
Journal:
J Cell Biol
Year:
2017
Type:
Article
Affiliation country:
United kingdom