Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM<sub>197</sub>.

Roth, R; van Zyl, P; Tsekoa, T; Stoychev, S; Mamputha, S; Buthelezi, S; Crampton, M

Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM₁₉₇.

Roth, R; van Zyl, P; Tsekoa, T; Stoychev, S; Mamputha, S; Buthelezi, S; Crampton, M.

Affiliation

Roth R; Biosciences, Council for Scientific and Industrial Research, Pretoria, South Africa.
van Zyl P; Biosciences, Council for Scientific and Industrial Research, Pretoria, South Africa.
Tsekoa T; Biosciences, Council for Scientific and Industrial Research, Pretoria, South Africa.
Stoychev S; Biosciences, Council for Scientific and Industrial Research, Pretoria, South Africa.
Mamputha S; Biosciences, Council for Scientific and Industrial Research, Pretoria, South Africa.
Buthelezi S; Biosciences, Council for Scientific and Industrial Research, Pretoria, South Africa.
Crampton M; Biosciences, Council for Scientific and Industrial Research, Pretoria, South Africa.

J Appl Microbiol ; 122(5): 1402-1411, 2017 May.

Article in En | MEDLINE | ID: mdl-28276616

Subject(s)

Bacterial Proteins/biosynthesis; Escherichia coli Proteins/genetics; Escherichia coli/metabolism; Gene Expression; Oxidoreductases/genetics; Protein Disulfide-Isomerases/genetics; Recombinant Proteins/biosynthesis; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins/metabolism; Oxidoreductases/metabolism; Protein Disulfide-Isomerases/metabolism; Protein Folding; Recombinant Proteins/chemistry; Recombinant Proteins/genetics

Key words

E. coli; cross-reacting material 197; disulphide bond formation; protein disulphide isomerase; soluble production; structural integrity; sulphydryl oxidase

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oxidoreductases / Bacterial Proteins / Recombinant Proteins / Gene Expression / Protein Disulfide-Isomerases / Escherichia coli Proteins / Escherichia coli Language: En Journal: J Appl Microbiol Journal subject: MICROBIOLOGIA Year: 2017 Type: Article Affiliation country: South Africa

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