A tissue-type plasminogen activator mutant with prolonged clearance in vivo. Effect of removal of the growth factor domain.
J Biol Chem
; 263(4): 1599-602, 1988 Feb 05.
Article
in En
| MEDLINE
| ID: mdl-2828346
ABSTRACT
The complete cDNA for human tissue-type plasminogen activator (t-PA) was cloned and sequenced. A mutant was constructed by using in vitro site-specific mutagenesis to delete the region encoding the growth factor domain (amino acids 51-87 inclusive). Normal and mutant t-PA species were produced using two mammalian expression systems (in human HeLa cells and mouse C127 cells). The clearance of mutant and normal t-PA from plasma was examined in vivo using a guinea pig model. Mutant t-PA derived from HeLa or C127 cells was cleared much more slowly than the cognate normal t-PA. The potential role of the growth factor domain in the recognition of t-PA by the hepatic clearance mechanism is discussed.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacterial Proteins
/
Deoxyribonucleases, Type II Site-Specific
/
Tissue Plasminogen Activator
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
J Biol Chem
Year:
1988
Type:
Article
Affiliation country:
United kingdom