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Optogenetic control with a photocleavable protein, PhoCl.
Zhang, Wei; Lohman, Alexander W; Zhuravlova, Yevgeniya; Lu, Xiaocen; Wiens, Matthew D; Hoi, Hiofan; Yaganoglu, Sine; Mohr, Manuel A; Kitova, Elena N; Klassen, John S; Pantazis, Periklis; Thompson, Roger J; Campbell, Robert E.
Affiliation
  • Zhang W; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Lohman AW; Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada.
  • Zhuravlova Y; Department of Cell Biology and Anatomy, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
  • Lu X; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Wiens MD; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Hoi H; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Yaganoglu S; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Mohr MA; Department of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland.
  • Kitova EN; Department of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland.
  • Klassen JS; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Pantazis P; Alberta Glycomics Centre, University of Alberta, Edmonton, Alberta, Canada.
  • Thompson RJ; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Campbell RE; Alberta Glycomics Centre, University of Alberta, Edmonton, Alberta, Canada.
Nat Methods ; 14(4): 391-394, 2017 Apr.
Article in En | MEDLINE | ID: mdl-28288123
ABSTRACT
To expand the range of experiments that are accessible with optogenetics, we developed a photocleavable protein (PhoCl) that spontaneously dissociates into two fragments after violet-light-induced cleavage of a specific bond in the protein backbone. We demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase, Gal4 transcription factor, and a viral protease that in turn was used to activate opening of the large-pore ion channel Pannexin-1.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Proteins / Protein Engineering / Optogenetics Limits: Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2017 Type: Article Affiliation country: Canada
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Proteins / Protein Engineering / Optogenetics Limits: Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2017 Type: Article Affiliation country: Canada