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HIV-1 susceptibility of transgenic rat-derived primary macrophage/T cells and a T cell line that express human receptors, CyclinT1 and CRM1 genes.
Shida, Hisatoshi; Okada, Hiroyuki; Suzuki, Hajime; Zhang, Xianfeng; Chen, Jing; Tsunetsugu-Yokota, Yasuko; Tanaka, Yuetsu; Yakushiji, Fumika; Hayashi, Yoshio.
Affiliation
  • Shida H; Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, 060-0815, Japan.
  • Okada H; Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, 060-0815, Japan.
  • Suzuki H; Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, 060-0815, Japan.
  • Zhang X; Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, 060-0815, Japan.
  • Chen J; Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, 060-0815, Japan.
  • Tsunetsugu-Yokota Y; Department of Medical Technology, Tokyo University of Technology, 5-23-22 Nishikamata, Ohta-ku, Tokyo, 144-8535, Japan.
  • Tanaka Y; Department of Immunology, Graduate School of Medicine, University of the Ryukyus, Uehara 207, Nishihara-cho, Okinawa, 903-0125, Japan.
  • Yakushiji F; Department of Medicinal Chemistry, Tokyo University of Pharmacy and Life Science, 1432-1, Horinouchi, Hachioji, Tokyo, 192-0392, Japan.
  • Hayashi Y; Department of Medicinal Chemistry, Tokyo University of Pharmacy and Life Science, 1432-1, Horinouchi, Hachioji, Tokyo, 192-0392, Japan.
Genes Cells ; 22(5): 424-435, 2017 May.
Article in En | MEDLINE | ID: mdl-28326644
ABSTRACT
We developed transgenic (Tg) rats that express human CD4, CCR5, CXCR4, CyclinT1, and CRM1 genes. Tg rat macrophages were efficiently infected with HIV-1 and supported production of infectious progeny virus. By contrast, both rat primary CD4+ T cells and established T cell lines expressing human CD4, CCR5, CyclinT1, and CRM1 genes were infected inefficiently, but this was ameliorated by inhibition of cyclophilin A. The infectivity of rat T cell-derived virus was lower than that of human T cell-derived virus.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: CD4-Positive T-Lymphocytes / HIV Infections / Receptors, Cytoplasmic and Nuclear / Karyopherins / Cyclin T / Macrophages Limits: Animals / Humans Language: En Journal: Genes Cells Journal subject: BIOLOGIA MOLECULAR Year: 2017 Type: Article Affiliation country: Japan
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: CD4-Positive T-Lymphocytes / HIV Infections / Receptors, Cytoplasmic and Nuclear / Karyopherins / Cyclin T / Macrophages Limits: Animals / Humans Language: En Journal: Genes Cells Journal subject: BIOLOGIA MOLECULAR Year: 2017 Type: Article Affiliation country: Japan