Quantitative identification of senescent cells in aging and disease.

Biran, Anat; Zada, Lior; Abou Karam, Paula; Vadai, Ezra; Roitman, Lior; Ovadya, Yossi; Porat, Ziv; Krizhanovsky, Valery

Biran, Anat; Zada, Lior; Abou Karam, Paula; Vadai, Ezra; Roitman, Lior; Ovadya, Yossi; Porat, Ziv; Krizhanovsky, Valery.

Affiliation

Biran A; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 76100, Israel.
Zada L; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 76100, Israel.
Abou Karam P; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 76100, Israel.
Vadai E; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 76100, Israel.
Roitman L; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 76100, Israel.
Ovadya Y; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 76100, Israel.
Porat Z; Flow Cytometry Unit, Biological Services Department, Weizmann Institute of Science, 76100, Rehovot, Israel.
Krizhanovsky V; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 76100, Israel.

Aging Cell ; 16(4): 661-671, 2017 08.

Article in En | MEDLINE | ID: mdl-28455874

Subject(s)

Aging/genetics; Cellular Senescence/genetics; Neoplasms/genetics; Single-Cell Analysis/methods; Staining and Labeling/methods; Aging/metabolism; Aging/pathology; Animals; Biomarkers/analysis; Cellular Senescence/drug effects; Epithelial Cells/metabolism; Epithelial Cells/pathology; Etoposide/pharmacology; Fibrosis; Flow Cytometry; Gene Expression; HMGB1 Protein/genetics; HMGB1 Protein/metabolism; Histones/genetics; Histones/metabolism; Humans; Image Processing, Computer-Assisted; Lymphocytes/metabolism; Lymphocytes/pathology; Mice; Molecular Imaging; Neoplasms/metabolism; Neoplasms/pathology; Primary Cell Culture; Stromal Cells/metabolism; Stromal Cells/pathology; beta-Galactosidase/genetics; beta-Galactosidase/metabolism

Key words

ImageStreamX; aging; cancer; cellular senescence; flow cytometry; senescence-associated beta-galactosidase

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Staining and Labeling / Aging / Cellular Senescence / Single-Cell Analysis / Neoplasms Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals / Humans Language: En Journal: Aging Cell Year: 2017 Type: Article Affiliation country: Israel

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