Uptake, transport and distribution of molybdenum in two oilseed rape (Brassica napus L.) cultivars under different nitrate/ammonium ratios.

ABSTRACT

OBJECTIVES: To investigate the effects of different nitrate sources on the uptake, transport, and distribution of molybdenum (Mo) between two oilseed rape (Brassica napus L.) cultivars, L0917 and ZS11. METHODS: A hydroponic culture experiment was conducted with four nitrate/ammonium (NO3-NH4+) ratios (141, 96, 7.57.5, and 114) at a constant nitrogen concentration of 15 mmol/L. We examined Mo concentrations in roots, shoots, xylem and phloem sap, and subcellular fractions of leaves to contrast Mo uptake, transport, and subcellular distribution between ZS11 and L0917. RESULTS: Both the cultivars showed maximum biomass and Mo accumulation at the 7.57.5 ratio of NO3-NH4+ while those were decreased by the 141 and 114 treatments. However, the percentages of root Mo (14.8% and 15.0% for L0917 and ZS11, respectively) were low under the 7.57.5 treatment, suggesting that the equal NO3-NH4+ ratio promoted Mo transportation from root to shoot. The xylem sap Mo concentration and phloem sap Mo accumulation of L0917 were lower than those of ZS11 under the 114 treatment, which suggests that higher NO3-NH4+ ratio was more beneficial for L0917. On the contrary, a lower NO3-NH4+ ratio was more beneficial for ZS11 to transport and remobilize Mo. Furthermore, the Mo concentrations of both the cultivars' leaf organelles were increased but the Mo accumulations of the cell wall and soluble fraction were reduced significantly under the 141 treatment, meaning that more Mo was accumulated in organelles under the highest NO3-NH4+ ratio. CONCLUSIONS: This investigation demonstrated that the capacities of Mo absorption, transportation and subcellular distribution play an important role in genotype-dependent differences in Mo accumulation under low or high NO3-NH4+ ratio conditions.