The performance of a resazurin chromogenic agar plate with a combined disc method for rapid screening of extended-spectrum-ß-lactamases, AmpC ß-lactamases and co-ß-lactamases in Enterobacteriaceae.
Microbiol Immunol
; 61(8): 297-304, 2017 Aug.
Article
in En
| MEDLINE
| ID: mdl-28685856
ABSTRACT
A promising means of rapid screening of extended-spectrum-ß-lactamase (ESBL), AmpC ß-lactamase, and co-production of ESBL and AmpC that combines resazurin chromogenic agar (RCA) with a combined disc method is here reported. Cefpodoxime (CPD) discs with and without clavulanic acid (CA), cloxacillin (CX) and CA+CX were evaluated against 86 molecularly confirmed ß-lactamase-producing Enterobacteriaceae, including 15 ESBLs, 32 AmpCs, nine co-producers of ESBL and AmpC and 30 carbapenemase producers. The CA and CX synergy test successfully detected all ESBL producers (100% sensitivity and 98.6% specificity) and all AmpC producers (100% sensitivity and 96.36% specificity). This assay also performed well in screening for co-existence of ESBL and AmpC (88.89% sensitivity and 100% specificity). The RCA assay is simple and inexpensive and provides results within 7 hr. It can be performed in any microbiological laboratory, in particular, in geographic regions in which ESBL, AmpC or co-ß-lactamase-producing Enterobacteriaceae are endemic.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oxazines
/
Bacterial Proteins
/
Xanthenes
/
Beta-Lactamases
/
Enterobacteriaceae
/
Disk Diffusion Antimicrobial Tests
/
Indicators and Reagents
/
Anti-Bacterial Agents
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Humans
Language:
En
Journal:
Microbiol Immunol
Year:
2017
Type:
Article
Affiliation country:
United kingdom