Evaluation of DNA methylation of inflammatory genes following treatment of chronic periodontitis: A pilot case-control study.
J Clin Periodontol
; 44(9): 905-914, 2017 Sep.
Article
in En
| MEDLINE
| ID: mdl-28736819
ABSTRACT
OBJECTIVE:
To evaluate the influence of periodontal therapy on DNA methylation in patients with chronic periodontitis as compared to healthy individuals. MATERIAL ANDMETHODS:
Twenty patients were enrolled into two groups (i) 10 diagnosed as clinically healthy; and (ii) 10 diagnosed with chronic periodontitis. Clinical measures were recorded and gingival biopsies were harvested at baseline (both patient groups) and at 2 and 8 weeks post-baseline for diseased individuals. Molecular DNA methylation analysis was performed by pyrosequencing for the putative inflammation-associated genes LINE-1, COX-2, IFN-γ and TNF-α. Random-intercept linear regression models were applied to evaluate methylation levels across groups at baseline and the methylation changes over time in the diseased and normal tissues.RESULTS:
Periodontal therapy did not influence gene expression methylation of TNF-α, IFN-γ and LINE-1 levels at normal and periodontitis sites over time. However, it significantly reduced COX-2 methylation levels comparable to healthy individuals at both 2 and 8 weeks post-treatment (p < .05).CONCLUSIONS:
Periodontal therapy resets the DNA methylation status of inflammatory gene for COX-2 in patients with periodontal disease. DNA methylation levels of TNF-α, IFN-γ and LINE-1 were sustained in periodontitis sites despite therapy. Future studies should consider an expanded panel of inflammatory genes over time. (ClinicalTrials.gov NCT02835898).Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA Methylation
/
Chronic Periodontitis
Type of study:
Clinical_trials
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Adult
/
Aged
/
Female
/
Humans
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Male
/
Middle aged
Language:
En
Journal:
J Clin Periodontol
Year:
2017
Type:
Article
Affiliation country:
Italy