A fixation method to preserve cultured cell cytonemes facilitates mechanistic interrogation of morphogen transport.

Bodeen, William J; Marada, Suresh; Truong, Ashley; Ogden, Stacey K

Bodeen, William J; Marada, Suresh; Truong, Ashley; Ogden, Stacey K.

Affiliation

Bodeen WJ; Department of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.
Marada S; Integrated Program in Biomedical Sciences, University of Tennessee Health Sciences Center, Memphis, TN, 38163, USA.
Truong A; Department of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.
Ogden SK; Department of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.

Development ; 144(19): 3612-3624, 2017 10 01.

Article in En | MEDLINE | ID: mdl-28827391

Subject(s)

Drosophila Proteins/metabolism; Pseudopodia/metabolism; Tissue Fixation/methods; Animals; Biological Transport; Cells, Cultured; Cholesterol/metabolism; Drosophila melanogaster/metabolism; Mice; NIH 3T3 Cells; Reproducibility of Results

Key words

Cytoneme; Dispatched; Hedgehog; Morphogen; Signal transduction

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pseudopodia / Tissue Fixation / Drosophila Proteins Type of study: Prognostic_studies Limits: Animals Language: En Journal: Development Journal subject: BIOLOGIA / EMBRIOLOGIA Year: 2017 Type: Article Affiliation country: United States

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