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A cerebrospinal fluid microRNA signature as biomarker for glioblastoma.
Akers, Johnny C; Hua, Wei; Li, Hongying; Ramakrishnan, Valya; Yang, Zixiao; Quan, Kai; Zhu, Wei; Li, Jie; Figueroa, Javier; Hirshman, Brian R; Miller, Brittney; Piccioni, David; Ringel, Florian; Komotar, Ricardo; Messer, Karen; Galasko, Douglas R; Hochberg, Fred; Mao, Ying; Carter, Bob S; Chen, Clark C.
Affiliation
  • Akers JC; Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA.
  • Hua W; Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
  • Li H; Biostatistics Department, Moores Cancer Center, UC San Diego Health System, La Jolla, CA, USA.
  • Ramakrishnan V; Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA.
  • Yang Z; Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
  • Quan K; Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
  • Zhu W; Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
  • Li J; Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA.
  • Figueroa J; Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA.
  • Hirshman BR; Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA.
  • Miller B; Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA.
  • Piccioni D; Department of Neurosurgery, Moores Cancer Center, UC San Diego Health System, La Jolla, CA, USA.
  • Ringel F; Department of Neurosurgery, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
  • Komotar R; Department of Neurological Surgery, Miller School of Medicine, University of Miami, Miami, FL, USA.
  • Messer K; Biostatistics Department, Moores Cancer Center, UC San Diego Health System, La Jolla, CA, USA.
  • Galasko DR; Department of Neurosciences, University of California, San Diego, CA, USA.
  • Hochberg F; Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA.
  • Mao Y; Department of Neurosciences, University of California, San Diego, CA, USA.
  • Carter BS; State Key Laboratory of Medical Neurobiology, Institutes of Brain Science, The Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, China.
  • Chen CC; Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA.
Oncotarget ; 8(40): 68769-68779, 2017 Sep 15.
Article in En | MEDLINE | ID: mdl-28978155
ABSTRACT

PURPOSE:

To develop a cerebrospinal fluid (CSF) miRNA diagnostic biomarker for glioblastoma. EXPERIMENTAL

DESIGN:

Glioblastoma tissue and matched CSF from the same patient (obtained prior to tumor manipulation) were profiled by TaqMan OpenArray® Human MicroRNA Panel. CSF miRNA profiles from glioblastoma patients and controls were created from three discovery cohorts and confirmed in two validation cohorts.

RESULTS:

miRNA profiles from clinical CSF correlated with those found in glioblastoma tissues. Comparison of CSF miRNA profiles between glioblastoma patients and non-brain tumor patients yielded a tumor "signature" consisting of nine miRNAs. The "signature" correlated with glioblastoma tumor volume (p=0.008). When prospectively applied to cisternal CSF, the sensitivity and specificity of the 'signature' for glioblastoma detection were 67% and 80%, respectively. For lumbar CSF, the sensitivity and specificity of the signature were 28% and 95%, respectively. Comparable results were obtained from analyses of CSF extracellular vesicles (EVs) and crude CSF.

CONCLUSION:

We report a CSF miRNA signature as a "liquid biopsy" diagnostic platform for glioblastoma.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Oncotarget Year: 2017 Type: Article Affiliation country: United States
Fulltext
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Oncotarget Year: 2017 Type: Article Affiliation country: United States