Safety and Accuracy of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Highly Pathogenic Organisms.
J Clin Microbiol
; 55(12): 3513-3529, 2017 12.
Article
in En
| MEDLINE
| ID: mdl-29021156
ABSTRACT
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) sample preparation methods, including the direct, on-plate formic acid, and ethanol/formic acid tube extraction methods, were evaluated for their ability to render highly pathogenic organisms nonviable and safe for handling in a biosafety level 2 laboratory. Of these, the tube extraction procedure was the most successful, with none of the tested strains surviving this sample preparation method. Tube extracts from several agents of bioterrorism and their near neighbors were analyzed in an eight-laboratory study to examine the utility of the Bruker Biotyper and Vitek MS MALDI-TOF MS systems and their in vitro diagnostic (IVD), research-use-only, and Security-Relevant databases, as applicable, to accurately identify these agents. Forty-six distinct strains of Bacillus anthracis, Yersinia pestis, Francisella tularensis, Burkholderia mallei, Burkholderia pseudomallei, Clostridium botulinum, Brucella melitensis, Brucella abortus, Brucella suis, and Brucella canis were extracted and distributed to participating laboratories for analysis. A total of 35 near-neighbor isolates were also analyzed.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Specimen Handling
/
Bacteria
/
Occupational Exposure
/
Bacteriological Techniques
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Microbial Viability
Type of study:
Clinical_trials
/
Diagnostic_studies
/
Evaluation_studies
Limits:
Humans
Language:
En
Journal:
J Clin Microbiol
Year:
2017
Type:
Article
Affiliation country:
United States