Development and characterization of novel chimeric monoclonal antibodies for broad spectrum neutralization of rabies virus.
PLoS One
; 12(10): e0186380, 2017.
Article
in En
| MEDLINE
| ID: mdl-29045436
ABSTRACT
Current post-exposure prophylaxis for rabies virus infection has several limitations in terms of supply, cost, safety, and efficacy. Attempts to replace human or equine rabies immune globulins (HRIG or ERIG) have been made by several companies and institutes. We developed potent monoclonal antibodies to neutralize a broad spectrum of rabies viruses by screening hybridomas received from the U.S. Centers for Disease Control and Prevention (CDC). Two kinds of chimeric human antibodies (chimeric #7 and #17) were constructed by cloning the variable regions from selected hybridomas and the constant region of a human antibody. Two antibodies were bound to antigenic site III and I/IV, respectively, and were able to neutralize 51 field isolates of rabies virus that were isolated at different times and places such as Asia, Africa, North America, South America, and Australia. These two antibodies neutralize rabies viruses with high efficacy in an in vivo test using Syrian hamster and mouse models and show low risk for adverse immunogenicity.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rabies
/
Rabies virus
/
Antibodies, Neutralizing
/
Antibodies, Monoclonal
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Country/Region as subject:
Africa
/
America do norte
/
America do sul
/
Asia
/
Oceania
Language:
En
Journal:
PLoS One
Journal subject:
CIENCIA
/
MEDICINA
Year:
2017
Type:
Article