Shewanella carassii sp. nov., isolated from surface swabs of crucian carp and faeces of a diarrhoea patient.
Int J Syst Evol Microbiol
; 67(12): 5284-5289, 2017 Dec.
Article
in En
| MEDLINE
| ID: mdl-29205124
ABSTRACT
Two strains, 08MAS2251T and LZ2016-166, were isolated from diverse samples in China collected from the surface of crucian carp and the faeces of a diarrhoea patient, respectively. Both strains were pink-orange coloured, Gram-negative, oxidase- and catalase-positive, facultative anaerobic and motile bacteria, produced H2S and reduced nitrates to nitrites. Growth occurred in the presence of 0-9â% (w/v) NaCl and at 10-42 °C. The optimum conditions were with 1â% (w/v) NaCl and at 35 °C. The phylogenetic tree of 16S rRNA gene demonstrated that strains 08MAS2251T and LZ2016-166 clustered in a distinctive clade next to the species Shewanella chilikensis JC5T within the genus Shewanella. Meanwhile, gyrB gene sequence analysis indicated that the two strains formed an independent branch that was clearly separate from all the other Shewanella species with sequence similarities from 68.49 to 95.74â%. The DNA G+C content of strain 08MAS2251T was 52.68 mol%. Genomic relatedness of in silico DNA-DNA hybridization between strain 08MAS2251T and phylogenetic neighbours ranged from 50.5-51.8â%, below the cutoff of 70â%. In addition, corresponding average nucleotide identity values were between 93.01 to 93.49%, which were lower than 95â% threshold. The major fatty acids of strain 08MAS2251T were C17ââ1ω8c (27.2â%), iso-C15ââ0 (22.5â%), summed feature 3 (C16ââ1ω6c and/or C16ââ1ω7c; 8.7â%), C16ââ0 (6.2â%), iso-C13ââ0 (5.6â%) and C17ââ0 (4.5â%). Based on phenotypic and genetic analysis, strains 08MAS2251T and LZ2016-166 are identified as a novel species of the genus Shewanella, for which the name Shewanellacarassii sp. nov. is proposed. The type strain is 08MAS2251T (=DSM 104682T=CGMCC 1.16033T).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phylogeny
/
Carps
/
Shewanella
/
Diarrhea
Limits:
Animals
/
Humans
/
Newborn
Country/Region as subject:
Asia
Language:
En
Journal:
Int J Syst Evol Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2017
Type:
Article