Multiple substitutions lead to increased loop flexibility and expanded specificity in <i>Acinetobacter baumannii</i> carbapenemase OXA-239.

Harper, Thomas M; June, Cynthia M; Taracila, Magdalena A; Bonomo, Robert A; Powers, Rachel A; Leonard, David A

Multiple substitutions lead to increased loop flexibility and expanded specificity in Acinetobacter baumannii carbapenemase OXA-239.

Harper, Thomas M; June, Cynthia M; Taracila, Magdalena A; Bonomo, Robert A; Powers, Rachel A; Leonard, David A.

Affiliation

Harper TM; Department of Chemistry, Grand Valley State University, Allendale, MI 49401, U.S.A.
June CM; Department of Chemistry, Grand Valley State University, Allendale, MI 49401, U.S.A.
Taracila MA; Department of Medicine, Case Western Reserve University, Cleveland, OH 44106, U.S.A.
Bonomo RA; Research Service, Louis Stokes Veterans Affairs Medical Center, Cleveland, OH 44106, U.S.A.
Powers RA; Department of Medicine, Case Western Reserve University, Cleveland, OH 44106, U.S.A.
Leonard DA; Research Service, Louis Stokes Veterans Affairs Medical Center, Cleveland, OH 44106, U.S.A.

Biochem J ; 475(1): 273-288, 2018 01 11.

Article in En | MEDLINE | ID: mdl-29229762

Subject(s)

Acinetobacter baumannii/enzymology; Amino Acid Substitution; Bacterial Proteins/chemistry; Carbapenems/chemistry; Cefotaxime/chemistry; Imipenem/chemistry; beta-Lactamases/chemistry; Acinetobacter baumannii/drug effects; Acinetobacter baumannii/genetics; Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Carbapenems/metabolism; Carbapenems/pharmacology; Catalytic Domain; Cefotaxime/metabolism; Cefotaxime/pharmacology; Cloning, Molecular; Crystallography, X-Ray; Doripenem; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression; Genetic Vectors/chemistry; Genetic Vectors/metabolism; Imipenem/metabolism; Imipenem/pharmacology; Kinetics; Models, Molecular; Mutation; Protein Binding; Protein Conformation, alpha-Helical; Protein Conformation, beta-Strand; Protein Interaction Domains and Motifs; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Substrate Specificity; beta-Lactamases/genetics; beta-Lactamases/metabolism

Key words

carbapemase; cefotaxime; doripenem; imipenem; ß-lactamase

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Beta-Lactamases / Carbapenems / Cefotaxime / Imipenem / Amino Acid Substitution / Acinetobacter baumannii Language: En Journal: Biochem J Year: 2018 Type: Article Affiliation country: United States

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