Structural determinant of the species-specific transcription of the mouse rRNA gene promoter.
Mol Cell Biol
; 9(1): 349-53, 1989 Jan.
Article
in En
| MEDLINE
| ID: mdl-2927396
ABSTRACT
Mammalian ribosomal DNA (rDNA) transcription has a certain species specificity such that, both in vivo and in vitro, human rDNA cannot be transcribed by mouse machinery and vice versa. This is due to a species-dependent transcription factor, TFID (Y. Mishima, I. Financsek, R. Kominami, and M. Muramatsu, Nucleic Acids Res. 106659-6670, 1982). On the basis of the information obtained from 5' and 3' substitution mutants, we prepared a chimeric gene in which the mouse sequence from positions -32 to -14 was inserted into the corresponding location of the human rDNA promoter. The chimeric gene could be transcribed by mouse extracts nearly as efficiently as the wild-type mouse promoter. The chimeric gene could also sequester transcription factor TFID at an efficiency similar to that for the mouse promoter. Partially purified mouse TFID that could not protect the human rDNA promoter against DNase I produced a clear footprint on this chimeric gene that was similar to that on mouse rDNA promoter. The basic structure of the mouse rDNA core promoter is discussed in relation to the interaction with TFID.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription Factors
/
Transcription, Genetic
/
DNA, Ribosomal
/
Promoter Regions, Genetic
Limits:
Animals
/
Humans
Language:
En
Journal:
Mol Cell Biol
Year:
1989
Type:
Article
Affiliation country:
Japan