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Evaluation of spectral libraries and sample preparation for DIA-LC-MS analysis of host cell proteins: A case study of a bacterially expressed recombinant biopharmaceutical protein.
Heissel, Søren; Bunkenborg, Jakob; Kristiansen, Max Per; Holmbjerg, Anne Fich; Grimstrup, Marie; Mørtz, Ejvind; Kofoed, Thomas; Højrup, Peter.
Affiliation
  • Heissel S; Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
  • Bunkenborg J; Alphalyse A/S, 5220 Odense SØ, Denmark.
  • Kristiansen MP; Statens Seruminstitut, 2300 Copenhagen S, Denmark.
  • Holmbjerg AF; Statens Seruminstitut, 2300 Copenhagen S, Denmark.
  • Grimstrup M; Alphalyse A/S, 5220 Odense SØ, Denmark.
  • Mørtz E; Alphalyse A/S, 5220 Odense SØ, Denmark.
  • Kofoed T; Alphalyse A/S, 5220 Odense SØ, Denmark.
  • Højrup P; Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark. Electronic address: php@bmb.sdu.dk.
Protein Expr Purif ; 147: 69-77, 2018 07.
Article in En | MEDLINE | ID: mdl-29526817
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / Biological Products / Recombinant Proteins / Proteins / Chromatography, Liquid / Tandem Mass Spectrometry Limits: Humans Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2018 Type: Article Affiliation country: Denmark
Fulltext
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / Biological Products / Recombinant Proteins / Proteins / Chromatography, Liquid / Tandem Mass Spectrometry Limits: Humans Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2018 Type: Article Affiliation country: Denmark