Sperm DNA fragmentation in the total and vital fractions before and after density gradient centrifugation: Significance in male fertility diagnosis.

Punjabi, U; Van Mulders, H; Goovaerts, I; Peeters, K; Clasen, K; Janssens, P; Zemtsova, O; De Neubourg, D

Punjabi, U; Van Mulders, H; Goovaerts, I; Peeters, K; Clasen, K; Janssens, P; Zemtsova, O; De Neubourg, D.

Affiliation

Punjabi U; Centre for Reproductive Medicine, Antwerp University Hospital, Wilrijkstraat 10, 2650 Edegem, Belgium. Electronic address: usha.punjabi@uza.be.
Van Mulders H; Centre for Reproductive Medicine, Antwerp University Hospital, Wilrijkstraat 10, 2650 Edegem, Belgium.
Goovaerts I; Centre for Reproductive Medicine, Antwerp University Hospital, Wilrijkstraat 10, 2650 Edegem, Belgium.
Peeters K; Centre for Reproductive Medicine, Antwerp University Hospital, Wilrijkstraat 10, 2650 Edegem, Belgium.
Clasen K; Centre for Reproductive Medicine, Antwerp University Hospital, Wilrijkstraat 10, 2650 Edegem, Belgium.
Janssens P; Centre for Reproductive Medicine, Antwerp University Hospital, Wilrijkstraat 10, 2650 Edegem, Belgium.
Zemtsova O; Centre for Reproductive Medicine, Antwerp University Hospital, Wilrijkstraat 10, 2650 Edegem, Belgium.
De Neubourg D; Centre for Reproductive Medicine, Antwerp University Hospital, Wilrijkstraat 10, 2650 Edegem, Belgium.

Clin Biochem ; 62: 47-54, 2018 Dec.

Article in En | MEDLINE | ID: mdl-29792832

Subject(s)

Cell Separation; DNA Fragmentation; Infertility, Male/pathology; Oxidative Stress; Semen Analysis/methods; Sperm Motility; Spermatozoa/pathology; Adolescent; Adult; Belgium/epidemiology; Cell Survival; Centrifugation, Density Gradient; Cohort Studies; Hospitals, University; Humans; In Situ Nick-End Labeling; Infertility, Male/diagnosis; Infertility, Male/epidemiology; Infertility, Male/physiopathology; Male; Middle Aged; Prevalence; Reproducibility of Results; Severity of Illness Index; Young Adult

Key words

Density gradient centrifugation; TUNEL assay; Total sperm DNA fragmentation; Vital sperm DNA fragmentation

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sperm Motility / Spermatozoa / Cell Separation / Oxidative Stress / DNA Fragmentation / Semen Analysis / Infertility, Male Type of study: Diagnostic_studies / Etiology_studies / Incidence_studies / Observational_studies / Prevalence_studies / Risk_factors_studies Limits: Adolescent / Adult / Humans / Male / Middle aged Country/Region as subject: Europa Language: En Journal: Clin Biochem Year: 2018 Type: Article

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