Dynamics and Spatial Genomics of the Nascent Transcriptome by Intron seqFISH.
Cell
; 174(2): 363-376.e16, 2018 07 12.
Article
in En
| MEDLINE
| ID: mdl-29887381
ABSTRACT
Visualization of the transcriptome and the nuclear organization in situ has been challenging for single-cell analysis. Here, we demonstrate a multiplexed single-molecule in situ method, intron seqFISH, that allows imaging of 10,421 genes at their nascent transcription active sites in single cells, followed by mRNA and lncRNA seqFISH and immunofluorescence. This nascent transcriptome-profiling method can identify different cell types and states with mouse embryonic stem cells and fibroblasts. The nascent sites of RNA synthesis tend to be localized on the surfaces of chromosome territories, and their organization in individual cells is highly variable. Surprisingly, the global nascent transcription oscillated asynchronously in individual cells with a period of 2 hr in mouse embryonic stem cells, as well as in fibroblasts. Together, spatial genomics of the nascent transcriptome by intron seqFISH reveals nuclear organizational principles and fast dynamics in single cells that are otherwise obscured.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
In Situ Hybridization, Fluorescence
/
Transcriptome
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Cell
Year:
2018
Type:
Article
Affiliation country:
United States