MIS416 as a siRNA Delivery System with the Ability to Target Antigen-Presenting Cells.
Nucleic Acid Ther
; 28(4): 225-232, 2018 08.
Article
in En
| MEDLINE
| ID: mdl-29893623
ABSTRACT
MIS416 is a microparticulate formulation derived from propionibacterium acnes cell wall skeletons with intrinsic adjuvant activity. Conjugates of MIS416-SS-peptide containing a disulfide linkage facilitate the cytoplasmic delivery and release of peptides in antigen-presenting cells (APCs). We hypothesized that MIS416-siRNA (small interfering RNA) conjugates, containing a disulfide linkage between MIS416 and the siRNA, would allow cytoplasmic release of siRNA in APCs. MIS416-SS-siStat3 conjugates added to cell culture medium of monolayers of DCs in culture flasks successfully targeted Stat3 mRNA in DCs in vitro without transfection, downregulating Stat3 mRNA and protein levels. These results suggest that MIS416-SS-siRNA conjugates can be used as a novel siRNA delivery system for the knockdown of mRNA levels in APCs.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genetic Therapy
/
Cancer Vaccines
/
RNA, Small Interfering
/
STAT3 Transcription Factor
Limits:
Humans
Language:
En
Journal:
Nucleic Acid Ther
Year:
2018
Type:
Article
Affiliation country:
New Zealand