[Therapeutic effects of Chai Qin Cheng Qi decoction on severe acute pancreatitis complicated liver damage in rats and its mechanisms].

ABSTRACT

OBJECTIVE: To study the effects of Chai Qin Cheng Qi Decoction(CQCQD)(Traditional Chinese Medicine)on severe acute pancreatitis (SAP)complicated liver damage in rats. METHODS: Seventy-two SD rats were randomly divided into three groups(n=24)Sham group, SAP group and CQCQD treatment group. SAP model was induced by retrograde injection with sodium taurocholate. The rats in CQCQD group received treatment with CQCQD. After modeling 1 h, 5 h, 10 h, pancreas and liver histopathological changes were observed. The serum levels of interleukin-6(IL-6), amylase(AMS), alanine aminotransferase(ALT) and aspartate transaminase(AST) were detected. IL-6 and monocyte chemotactic protein 1(MCP-1)mRNA in pancreas and liver were assayed. RESULTS: Compared with sham group, the activities of AMS,ALT and AST and the serum level of IL-6 in SAP group were increased significantly. The levels of MCP-1 and IL-6 mRNA in pancreas and liver tissue were increased (P<0.05). Compared with SAP group, the activities of AMS,ALT and AST and the level of IL-6 in CQCQD group were reduced significantly (P<0.05). The pancreas and liver tissue pathological damage alleviated. The levels of IL-6 and MCP-1mRNA in pancreas and liver were decreased significantly(P<0.05). CONCLUSIONS: MCP-1 takes part in the progression of SAP complicated liver damage; CQCQD can significantly inhibit the expression of pancreas and liver MCP-1, alleviate pathological damage of pancreas and liver in SAP and play a therapeutic role in SAP complicated liver damage.