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SMA1, a homolog of the splicing factor Prp28, has a multifaceted role in miRNA biogenesis in Arabidopsis.
Li, Shengjun; Xu, Ran; Li, Aixia; Liu, Kan; Gu, Liqing; Li, Mu; Zhang, Hairui; Zhang, Yueying; Zhuang, Shangshang; Wang, Quanhui; Gao, Gang; Li, Na; Zhang, Chi; Li, Yunhai; Yu, Bin.
Affiliation
  • Li S; Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Engineering Research Center of Biomass Resources and Environment, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
  • Xu R; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
  • Li A; School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0118, USA.
  • Liu K; State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Centre for Excellence in Molecular Plant Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • Gu L; State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Centre for Excellence in Molecular Plant Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • Li M; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
  • Zhang H; School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0118, USA.
  • Zhang Y; State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Centre for Excellence in Molecular Plant Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • Zhuang S; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
  • Wang Q; School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0118, USA.
  • Gao G; School of Life Science, Shanxi Normal University, Linfen 041004, China.
  • Li N; State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Centre for Excellence in Molecular Plant Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • Zhang C; State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Centre for Excellence in Molecular Plant Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • Li Y; Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Engineering Research Center of Biomass Resources and Environment, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
  • Yu B; School of Life Science, Shanxi Normal University, Linfen 041004, China.
Nucleic Acids Res ; 46(17): 9148-9159, 2018 09 28.
Article in En | MEDLINE | ID: mdl-29982637
ABSTRACT
MicroRNAs (miRNAs) are a class of small non-coding RNAs that repress gene expression. In plants, the RNase III enzyme Dicer-like (DCL1) processes primary miRNAs (pri-miRNAs) into miRNAs. Here, we show that SMALL1 (SMA1), a homolog of the DEAD-box pre-mRNA splicing factor Prp28, plays essential roles in miRNA biogenesis in Arabidopsis. A hypomorphic sma1-1 mutation causes growth defects and reduces miRNA accumulation correlated with increased target transcript levels. SMA1 interacts with the DCL1 complex and positively influences pri-miRNA processing. Moreover, SMA1 binds the promoter region of genes encoding pri-miRNAs (MIRs) and is required for MIR transcription. Furthermore, SMA1 also enhances the abundance of the DCL1 protein levels through promoting the splicing of the DCL1 pre-mRNAs. Collectively, our data provide new insights into the function of SMA1/Prp28 in regulating miRNA abundance in plants.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arabidopsis / Arabidopsis Proteins / MicroRNAs / DEAD-box RNA Helicases Language: En Journal: Nucleic Acids Res Year: 2018 Type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arabidopsis / Arabidopsis Proteins / MicroRNAs / DEAD-box RNA Helicases Language: En Journal: Nucleic Acids Res Year: 2018 Type: Article Affiliation country: China