TIMP-1 association with collagen type I overproduction in hereditary gingival fibromatosis.
Oral Dis
; 24(8): 1581-1590, 2018 Nov.
Article
in En
| MEDLINE
| ID: mdl-29989318
ABSTRACT
OBJECTIVES:
To investigate the processes associated with the excessive production of collagen I in hereditary gingival fibromatosis (HGF). MATERIALS ANDMETHODS:
Three HGF subjects and five controls were enrolled in the study. Histomorphological and immunohistological analyses were performed on gingival tissues. The expression of heat-shock protein 47 (HSP47), collagen I, transforming growth factor-ß1 (TGF-ß1), connective tissue growth factor (CTGF), matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) by gingival fibroblasts isolated from HGF and controls was analysed using qRT-PCR, Western blotting and ELISA.RESULTS:
Considerable accumulation of fibrotic fibrils and increased synthesis of HSP47 were noted in HGF gingival tissues. The synthesis of collagen I, HSP47, TGF-ß1, CTGF and TIMP-1 was significantly elevated in HGF gingival fibroblasts compared with controls, while the production of MMP-1 was decreased.CONCLUSIONS:
We report that fibrosis in HGF gingival tissues is associated with increased synthesis of HSP47. This finding was confirmed by an in vitro study, where excessive production of collagen I was associated with increased synthesis of HSP47, TGF-ß1 and CTGF by HGF gingival fibroblasts. Moreover, the shift in the TIMP-1/MMP-1 ratio identifies increased synthesis of TIMP-1 as one of the processes associated with collagen I overproduction in HGF fibroblasts.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tissue Inhibitor of Metalloproteinase-1
/
Collagen Type I
/
HSP47 Heat-Shock Proteins
/
Fibromatosis, Gingival
Type of study:
Prognostic_studies
/
Risk_factors_studies
Limits:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
Language:
En
Journal:
Oral Dis
Journal subject:
ODONTOLOGIA
Year:
2018
Type:
Article
Affiliation country:
Poland