Baculovirus-Induced Fast-Acting Innate Immunity Kills Liver-Stage Plasmodium.
J Immunol
; 201(8): 2441-2451, 2018 10 15.
Article
in En
| MEDLINE
| ID: mdl-30209187
ABSTRACT
Baculovirus (BV), an enveloped insect virus with a circular dsDNA genome, possesses unique characteristics that induce strong innate immune responses in mammalian cells. In this study, we show that BV administration in BALB/c mice not only provides complete protection against a subsequent Plasmodium berghei sporozoite infection for up to 7 d after the injection but also eliminates existing liver-stage parasites completely. The elimination of sporozoites by BV was superior to that by primaquine, and this effect occurred in a TLR9-independent manner. At 6 h after BV administration, IFN-α and IFN-γ were robustly produced in the serum, and RNA transcripts of IFN-stimulated genes were markedly upregulated in the liver compared with control mice. The in vivo passive transfer of serum after BV administration effectively eliminated liver-stage parasites, and IFN-α neutralization abolished this effect, indicating that the BV liver-stage parasite-killing mechanism is downstream of the type I IFN signaling pathway. These findings provide evidence that BV-induced, fast-acting innate immunity completely kills liver-stage parasites and, thus, may lead to new malaria drug and vaccine strategies.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasmodium berghei
/
Baculoviridae
/
Immunotherapy, Adoptive
/
Malaria Vaccines
/
Liver
/
Malaria
Limits:
Animals
Language:
En
Journal:
J Immunol
Year:
2018
Type:
Article
Affiliation country:
Japan