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Expedited assessment of terrestrial arthropod diversity by coupling Malaise traps with DNA barcoding 1.
deWaard, Jeremy R; Levesque-Beaudin, Valerie; deWaard, Stephanie L; Ivanova, Natalia V; McKeown, Jaclyn T A; Miskie, Renee; Naik, Suresh; Perez, Kate H J; Ratnasingham, Sujeevan; Sobel, Crystal N; Sones, Jayme E; Steinke, Claudia; Telfer, Angela C; Young, Andrew D; Young, Monica R; Zakharov, Evgeny V; Hebert, Paul D N.
Affiliation
  • deWaard JR; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
  • Levesque-Beaudin V; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
  • deWaard SL; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
  • Ivanova NV; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
  • McKeown JTA; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
  • Miskie R; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
  • Naik S; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
  • Perez KHJ; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
  • Ratnasingham S; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
  • Sobel CN; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
  • Sones JE; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
  • Steinke C; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
  • Telfer AC; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
  • Young AD; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
  • Young MR; b Department of Biology, Carleton University, Ottawa, Ontario, Canada.
  • Zakharov EV; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
  • Hebert PDN; a Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
Genome ; 62(3): 85-95, 2019 Mar.
Article in En | MEDLINE | ID: mdl-30257096
ABSTRACT
Monitoring changes in terrestrial arthropod communities over space and time requires a dramatic increase in the speed and accuracy of processing samples that cannot be achieved with morphological approaches. The combination of DNA barcoding and Malaise traps allows expedited, comprehensive inventories of species abundance whose cost will rapidly decline as high-throughput sequencing technologies advance. Aside from detailing protocols from specimen sorting to data release, this paper describes their use in a survey of arthropod diversity in a national park that examined 21 194 specimens representing 2255 species. These protocols can support arthropod monitoring programs at regional, national, and continental scales.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arthropods / DNA / Biodiversity / Entomology / DNA Barcoding, Taxonomic Limits: Animals Language: En Journal: Genome Journal subject: GENETICA Year: 2019 Type: Article Affiliation country: Canada
Fulltext
Print
XML
PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arthropods / DNA / Biodiversity / Entomology / DNA Barcoding, Taxonomic Limits: Animals Language: En Journal: Genome Journal subject: GENETICA Year: 2019 Type: Article Affiliation country: Canada