[Epidemiological Survey of Thalassemia in Women of childbearing age in Quanzhou Area of Fujian Province in China].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 26(5): 1453-1458, 2018 Oct.
Article
in Zh
| MEDLINE
| ID: mdl-30295267
ABSTRACT
OBJECTIVE:
To investigate the gene prevalence and spectrum of thalassemia in the women of childbearing age in quanzhou area.METHODS:
Venous blood of the women were collected for study, all subjects were registered in each county of quanzhou area by using cluster sampling. Both the mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) were used for screening thalassemiaï¼Genotyping of the screened positive samples was performed by gap single polymerase chain reaction (gap-PCR) and reverse dot blot (RDB) hybridizationï¼Unknown positive samples were analyzed with DNA sequencing.RESULTS:
Out of all 7 082 samples, Three hundred and eighty four were identified as thalassemia gene carriers with a carrying rate of 5.42 %. The α and ß thalassemia were 3.21% and 2.15% respectively. --SEA /αα was the most common genotype with 68.72 % in mutation types of α thalassemia, In addition gene, 2 cases of --THAI/αα and 1 case of αα/αααanti3.7 were also detected. IVS-â ¡-654/N and CD41-42/N were the most common gentypes with 75.00 % in mutation types of ß thalassemia gene, 5 cases were found to be α ß compogite thalassemia.CONCLUSION:
The carrying rate of thalassemia gene in quanzhou area is higher, and with the most common gentypes including --SEA /ααãIVSâ ¡-654(CâT)/N and CD41-42(-TTCT)/N. The study results are beneficial for the screening of thalassemia in the genetic consultation and the prenatal gene diagnosis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Beta-Thalassemia
/
Alpha-Thalassemia
Type of study:
Risk_factors_studies
Limits:
Female
/
Humans
/
Pregnancy
Country/Region as subject:
Asia
Language:
Zh
Journal:
Zhongguo Shi Yan Xue Ye Xue Za Zhi
Journal subject:
HEMATOLOGIA
Year:
2018
Type:
Article
Affiliation country:
China