Your browser doesn't support javascript.
loading
Identification of DNA methylation regulated novel host genes relevant to inhibition of virus replication in porcine PK15 cell using double stranded RNA mimics and DNA methyltransferase inhibitor.
Wang, Xiaoshuo; Ao, Hong; Song, Minyan; Bai, Lijing; He, Weiyong; Wang, Chuduan; Yu, Ying.
Affiliation
  • Wang X; National Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics and Breeding, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing 100193, China; Department of Animal Husbandry and Veterinary
  • Ao H; State Key Laboratory for Animal Nutrition, Key Laboratory for Domestic Animal Genetic Resources and Breeding of the Ministry of Agriculture of China, Chinese Academy of Agricultural Sciences, Institute of Animal Science, Beijing 100193, China.
  • Song M; National Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics and Breeding, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing 100193, China.
  • Bai L; State Key Laboratory for Animal Nutrition, Key Laboratory for Domestic Animal Genetic Resources and Breeding of the Ministry of Agriculture of China, Chinese Academy of Agricultural Sciences, Institute of Animal Science, Beijing 100193, China.
  • He W; State Key Laboratory of Agrobiotechnology, Department of Microbiology and Immunology, College of Biological Science, China Agricultural University, Beijing 100193, China.
  • Wang C; National Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics and Breeding, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing 100193, China. Electronic address: cdwang@cau.edu.cn.
  • Yu Y; National Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics and Breeding, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing 100193, China. Electronic address: yuying@cau.edu.cn.
Genomics ; 111(6): 1464-1473, 2019 12.
Article in En | MEDLINE | ID: mdl-30315899
ABSTRACT
During RNA viruses's replication, double-stranded RNA (dsRNA) is normally produced and induce host innate immune response. Most of gene activation due cytokine mediated but which are due to methylation mediated is still unknown. In the study, DNA methylome was integrated with our previous transcriptome data to investigate the differentially methylated regions and genes using MeDIP-chip technology. We found that the transcriptional expressions of 15, 37 and 18 genes were negatively related with their promoter DNA methylation levels in the cells treated by PolyIC, Aza-CdR, as well as PolyIC plus Aza-CdR, respectively, compared with the untreated cells. GO analysis revealed hypo-methylated genes (BNIP3L and CDK9) and a hyper-methylated gene (ZC3HAV1) involved in the host response to viral replication. Our results suggest that these novel genes targeted by DNA methylation can be potential markers relevant to virus replication and host innate immune response to set up a medical model of infectious diseases.
Subject(s)
Key words
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Azacitidine / Virus Replication / RNA, Double-Stranded / DNA Modification Methylases / Gene Expression Regulation / DNA Methylation / Kidney Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Genomics Journal subject: GENETICA Year: 2019 Type: Article
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Azacitidine / Virus Replication / RNA, Double-Stranded / DNA Modification Methylases / Gene Expression Regulation / DNA Methylation / Kidney Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Genomics Journal subject: GENETICA Year: 2019 Type: Article