Identification of DNA methylation regulated novel host genes relevant to inhibition of virus replication in porcine PK15 cell using double stranded RNA mimics and DNA methyltransferase inhibitor.
Genomics
; 111(6): 1464-1473, 2019 12.
Article
in En
| MEDLINE
| ID: mdl-30315899
ABSTRACT
During RNA viruses's replication, double-stranded RNA (dsRNA) is normally produced and induce host innate immune response. Most of gene activation due cytokine mediated but which are due to methylation mediated is still unknown. In the study, DNA methylome was integrated with our previous transcriptome data to investigate the differentially methylated regions and genes using MeDIP-chip technology. We found that the transcriptional expressions of 15, 37 and 18 genes were negatively related with their promoter DNA methylation levels in the cells treated by PolyIC, Aza-CdR, as well as PolyIC plus Aza-CdR, respectively, compared with the untreated cells. GO analysis revealed hypo-methylated genes (BNIP3L and CDK9) and a hyper-methylated gene (ZC3HAV1) involved in the host response to viral replication. Our results suggest that these novel genes targeted by DNA methylation can be potential markers relevant to virus replication and host innate immune response to set up a medical model of infectious diseases.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Azacitidine
/
Virus Replication
/
RNA, Double-Stranded
/
DNA Modification Methylases
/
Gene Expression Regulation
/
DNA Methylation
/
Kidney
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Genomics
Journal subject:
GENETICA
Year:
2019
Type:
Article