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Efficient mRNA delivery system utilizing chimeric VSVG-L7Ae virus-like particles.
Zhitnyuk, Yulia; Gee, Peter; Lung, Mandy S Y; Sasakawa, Noriko; Xu, Huaigeng; Saito, Hirohide; Hotta, Akitsu.
Affiliation
  • Zhitnyuk Y; Center for Data-Intensive Biomedicine and Biotechnology, Skolkovo Institute of Science and Technology, Skolkovo, 143025, Russia.
  • Gee P; Department of Life Science Frontiers, Center for iPS cell Research and Application, Kyoto University, Kyoto, 606-8507, Japan.
  • Lung MSY; Department of Life Science Frontiers, Center for iPS cell Research and Application, Kyoto University, Kyoto, 606-8507, Japan.
  • Sasakawa N; Department of Life Science Frontiers, Center for iPS cell Research and Application, Kyoto University, Kyoto, 606-8507, Japan.
  • Xu H; Department of Life Science Frontiers, Center for iPS cell Research and Application, Kyoto University, Kyoto, 606-8507, Japan.
  • Saito H; Department of Life Science Frontiers, Center for iPS cell Research and Application, Kyoto University, Kyoto, 606-8507, Japan.
  • Hotta A; Department of Life Science Frontiers, Center for iPS cell Research and Application, Kyoto University, Kyoto, 606-8507, Japan. Electronic address: akitsu.hotta@cira.kyoto-u.ac.jp.
Biochem Biophys Res Commun ; 505(4): 1097-1102, 2018 11 10.
Article in En | MEDLINE | ID: mdl-30316514
ABSTRACT
The delivery of mRNA is advantageous over DNA delivery as it is transient and does not carry the risk of genomic DNA integration. However, there are currently few efficient mRNA delivery options available, especially for hard-to-transfect cell types, and thus new delivery methods are needed. To this end, we have established a novel mRNA delivery system utilizing chimeric virus-like particles (VLPs). We generated a novel VLP by fusing protein G of Vesicular stomatitis virus (VSV-G) with a ribosomal protein L7Ae of Archeoglobus fulgidus. This system allowed the efficient delivery of EGFP mRNA which was independent from the presence of BoxC/D motif in the mRNA sequence. Our VSVG-L7Ae VLP system demonstrated high transduction efficacy in hard-to-transfect cell lines, such as human induced pluripotent stem cells (iPS cells) and monocytes. In summary, this platform may serve as an efficient and transient transgene delivery tool for an mRNA of interest.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ribosomal Proteins / RNA, Messenger / Membrane Glycoproteins / Viral Envelope Proteins / Gene Transfer Techniques Limits: Humans Language: En Journal: Biochem Biophys Res Commun Year: 2018 Type: Article Affiliation country: RUSSIA
Fulltext
Print
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ribosomal Proteins / RNA, Messenger / Membrane Glycoproteins / Viral Envelope Proteins / Gene Transfer Techniques Limits: Humans Language: En Journal: Biochem Biophys Res Commun Year: 2018 Type: Article Affiliation country: RUSSIA