Improved yield of rhEPO in CHO cells with synthetic 5' UTR.

Costello, Alan; Lao, Nga T; Barron, Niall; Clynes, Martin

Costello, Alan; Lao, Nga T; Barron, Niall; Clynes, Martin.

Affiliation

Costello A; National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, D09 NR58, Ireland. alan.costello23@mail.dcu.ie.
Lao NT; National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, D09 NR58, Ireland.
Barron N; National Institute for Bioprocessing Research and Training, Dublin, A94 X099, Ireland.
Clynes M; University College Dublin, Belfield, Dublin 4, D04 V1W8, Ireland.

Biotechnol Lett ; 41(2): 231-239, 2019 Feb.

Article in En | MEDLINE | ID: mdl-30506229

Subject(s)

5' Untranslated Regions; Erythropoietin/genetics; RNA, Messenger/metabolism; Recombinant Proteins/metabolism; Adenosine/analogs & derivatives; Adenosine/metabolism; Animals; Batch Cell Culture Techniques; CHO Cells; Cricetulus; Erythropoietin/metabolism; Mutation; Protein Biosynthesis; RNA, Messenger/genetics; Recombinant Proteins/genetics; Synthetic Biology/methods

Key words

5' UTR; Bioprocessing; CHO; N 6-methyladenosine; Synthetic biology; m6A

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Proteins / RNA, Messenger / Erythropoietin / 5' Untranslated Regions Limits: Animals Language: En Journal: Biotechnol Lett Year: 2019 Type: Article Affiliation country: Ireland

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