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miR-222 represses expression of zipcode binding protein-1 and phospholipase C-γ1 in intestinal epithelial cells.
Jiang, Li-Ping; Wang, Shelley R; Chung, Hee Kyoung; Buddula, Saharsh; Wang, Jian-Ying; Rao, Jaladanki N.
Affiliation
  • Jiang LP; Cell Biology Group, Department of Surgery, University of Maryland School of Medicine , Baltimore, Maryland.
  • Wang SR; Cell Biology Group, Department of Surgery, University of Maryland School of Medicine , Baltimore, Maryland.
  • Chung HK; Baltimore Veterans Affairs Medical Center , Baltimore, Maryland.
  • Buddula S; Cell Biology Group, Department of Surgery, University of Maryland School of Medicine , Baltimore, Maryland.
  • Wang JY; Baltimore Veterans Affairs Medical Center , Baltimore, Maryland.
  • Rao JN; Cell Biology Group, Department of Surgery, University of Maryland School of Medicine , Baltimore, Maryland.
Am J Physiol Cell Physiol ; 316(3): C415-C423, 2019 03 01.
Article in En | MEDLINE | ID: mdl-30649922
Both zipcode binding protein-1 (ZBP1) and phospholipase C-γ1 (PLCγ1) are intimately involved in many aspects of early intestinal mucosal repair after acute injury, but the exact mechanisms that control their cellular abundances remain largely unknown. The present study shows that microRNA-222 (miR-222) interacts with the mRNAs encoding ZBP1 and PLCγ1 and regulates ZBP1 and PLCγ1 expression in intestinal epithelial cells (IECs). The biotinylated miR-222 bound specifically to the ZBP1 and PLCγ1 mRNAs in IECs. Ectopically expressed miR-222 precursor destabilized the ZBP1 and PLCγ1 mRNAs and consequently lowered the levels of cellular ZBP1 and PLCγ1 proteins. Conversely, decreasing the levels of cellular miR-222 by transfection with its antagonism increased the stability of the ZBP1 and PLCγ1 mRNAs and increased the levels of ZBP1 and PLCγ1 proteins. Overexpression of miR-222 also inhibited cell migration over the wounded area, which was partially abolished by overexpressing ZBP1 and PLCγ1. Furthermore, prevention of the increased levels of ZBP1 and PLCγ1 in the miR-222-silenced cells by transfection with specific small interfering RNAs targeting ZBP1 or PLCγ1 mRNA inhibited cell migration after wounding. These findings indicate that induced miR-222 represses expression of ZBP1 and PLCγ1 at the posttranscriptional level, thus inhibiting IEC migration during intestinal epithelial restitution after wounding.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: MicroRNAs / DNA-Binding Proteins / Epithelial Cells / Phospholipase C gamma / Intestinal Mucosa Limits: Humans Language: En Journal: Am J Physiol Cell Physiol Journal subject: FISIOLOGIA Year: 2019 Type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: MicroRNAs / DNA-Binding Proteins / Epithelial Cells / Phospholipase C gamma / Intestinal Mucosa Limits: Humans Language: En Journal: Am J Physiol Cell Physiol Journal subject: FISIOLOGIA Year: 2019 Type: Article