Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Chromium Release Assay.

van der Haar Àvila, Irene; Marmol, Patricia; Kiessling, Rolf; Pico de Coaña, Yago

van der Haar Àvila, Irene; Marmol, Patricia; Kiessling, Rolf; Pico de Coaña, Yago.

Affiliation

van der Haar Àvila I; Department of Oncology-Pathology, Karolinska Institute, Stockholm, Sweden.
Marmol P; Division of Tumor Biology and Immunology (B3), NKI-AvL, Amsterdam, The Netherlands.
Kiessling R; Department of Cellular and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
Pico de Coaña Y; Department of Oncology-Pathology, Karolinska Institute, Stockholm, Sweden.

Methods Mol Biol ; 1913: 167-179, 2019.

Article in En | MEDLINE | ID: mdl-30666606

Subject(s)

Chromium Radioisotopes/metabolism; Cytotoxicity Tests, Immunologic/methods; Killer Cells, Natural/immunology; Antibody-Dependent Cell Cytotoxicity/drug effects; Antibody-Dependent Cell Cytotoxicity/immunology; Breast Neoplasms/blood; Breast Neoplasms/immunology; Breast Neoplasms/pathology; Cell Line, Tumor; Cell Separation/instrumentation; Cell Separation/methods; Cytotoxicity Tests, Immunologic/instrumentation; Female; Flow Cytometry/instrumentation; Flow Cytometry/methods; GPI-Linked Proteins/immunology; GPI-Linked Proteins/metabolism; Humans; Killer Cells, Natural/metabolism; Receptor, ErbB-2/antagonists & inhibitors; Receptor, ErbB-2/metabolism; Receptors, IgG/immunology; Receptors, IgG/metabolism; Trastuzumab/pharmacology

Key words

Antibody cell-mediated cytotoxicity; Chromium release assay; Natural killer cells; Trastuzumab

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cytotoxicity Tests, Immunologic / Killer Cells, Natural / Chromium Radioisotopes Limits: Female / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2019 Type: Article Affiliation country: Sweden

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