[Microflora of peripheral blood obtained from patients with infective endocarditis.]

ABSTRACT

Infective endocarditis is a serious inflammatory disease associated with damage of heart valves and other parts of endocardium. This study included 91 patients with a confirmed diagnosis of «infectious endocarditis¼ and hospitalized at the Research Institute of Complex Problems of Cardiovascular Disease (Kemerovo, Russia) from 2010 to 2015. The determination of the spectrum of microorganisms in the samples of patients' peripheral blood was carried out using the PCR method using reagents produced by Lyteh Ltd. (Moscow, Russia) allowing detect Staphylococcus spp., Staphylococcus aureus, Streptococcus pyogenes, Streptococcus agalacticae, Enterobacter spp., Klebsiella spp., Proteus spp., Haemophilus influenza, Enterococcus faecalis, Enterococcus faecium in the samples of biological material. Statistical analysis was performed in the StatSoft STATISTICA 10 software. 83.5% of patients were characterized by presence Staphylococcus spp. in the peripheral blood; the other pathogens were detected much less often (from six cases of enterococci detection to one case of Streptococcus agalacticae and Proteus spp.). In nine patients, none of the analyzed pathogens was detected, and a number of patients were characterized by the simultaneous presence of several pathogens. There was no reliable data on the difference in microflora structure depending on sex, drug addiction, the type of infective endocarditis and the damaged valve. It was established that the results of PCR of peripheral blood samples and microbiological examination of the tissues of damaged valves that were removed during cardiac surgery, differ significantly. The obtained results testify to the need for more in-depth studies including PCR analysis of peripheral blood samples, flushing from damaged valves, and also homogenized valve tissue samples, which will allow us to obtain more detailed data and conclude that PCR can be used as a diagnostic test for early detection microbiological agent as causative.