Submolecular dissection reveals strong and specific binding of polyamide-pyridostatin conjugates to human telomere interface.
Nucleic Acids Res
; 47(7): 3295-3305, 2019 04 23.
Article
in En
| MEDLINE
| ID: mdl-30820532
ABSTRACT
To modulate biological functions, G-quadruplexes in genome are often non-specifically targeted by small molecules. Here, specificity is increased by targeting both G-quadruplex and its flanking duplex DNA in a naturally occurring dsDNA-ssDNA telomere interface using polyamide (PA) and pyridostatin (PDS) conjugates (PA-PDS). We innovated a single-molecule assay in which dissociation constant (Kd) of the conjugate can be separately evaluated from the binding of either PA or PDS. We found Kd of 0.8 nM for PA-PDS, which is much lower than PDS (Kd â¼ 450 nM) or PA (Kd â¼ 35 nM). Functional assays further indicated that the PA-PDS conjugate stopped the replication of a DNA polymerase more efficiently than PA or PDS. Our results not only established a new method to dissect multivalent binding into actions of individual monovalent components, they also demonstrated a strong and specific G-quadruplex targeting strategy by conjugating highly specific duplex-binding molecules with potent quadruplex ligands.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Picolinic Acids
/
Telomere
/
Aminoquinolines
/
Nylons
Limits:
Humans
Language:
En
Journal:
Nucleic Acids Res
Year:
2019
Type:
Article
Affiliation country:
United States