Coupling S-adenosylmethionine-dependent methylation to growth: Design and uses.
PLoS Biol
; 17(3): e2007050, 2019 03.
Article
in En
| MEDLINE
| ID: mdl-30856169
ABSTRACT
We present a selection design that couples S-adenosylmethionine-dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution. We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone. Implementation of this design in Saccharomyces cerevisiae is also demonstrated.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
S-Adenosylmethionine
/
Saccharomyces cerevisiae
/
Saccharomyces cerevisiae Proteins
Language:
En
Journal:
PLoS Biol
Journal subject:
BIOLOGIA
Year:
2019
Type:
Article
Affiliation country:
Denmark